Ilex Life Sciences FGF-2 (FGF-basic) Bovine (155 aa), E. coli Recombinant Protein (50 ug). DESCRIPTION: Our bioactive FGF-2 (FGF-basic) Bovine, E. coli Recombinant Protein (155 aa), also known as Fibroblast Growth Factor 2, is a single, non-glycosylated, polypeptide chain that contains 155 amino acids and has a 17250 Dalton molecular mass. APPLICATION: FGF basic is a critical component of hESCs culture medium; the growth factor is necessary for the cells to remain in an undifferentiated state. FORMULATION: Sterile filtered white powder lyophilized from a concentrated (1 mg/ml) sterile solution containing 1% HSA. PURITY: Greater than 97.0% (RP-HPLC; SDS-PAGE). BIOACTIVITY: The ED50, measured in a mitogenic assay using quiescent NR6R-3T3 fibroblasts was found to be <0.1 ng/ml, corresponding to a specific activity of 3 x 106 Units/mg. OTHER NAMES: FGF2, FGF-b, Basic fibroblast growth factor, bFGF, Heparin-binding growth factor 2, HBGF-2.